Contamination from amplified products is a major concern when working with microbiological based techniques. Both culture and PCR amplify the pathogen for the purpose of detection and usually require sterile technique or clean rooms. PathogINDICAtor™ is able to provide a contamination free technique through a proprietary process known as DREAM PCR which uses methylation specific restriction enzymes to prevent the carryover of amplified products from one reaction to the next.
In the event that any DNA products from previous reactions are unintentionally introduced to subsequent reactions, an on board DREAM PCR™ enzyme will digest any DNA contaminants from previous tests. Any contaminant will have been tagged with a DREAM PCR™ methylation profile specifically targeted by the enzyme. The DREAM PCR™ enzyme is then deactivated so that the present reaction can proceed normally.